Maik Haentschel*, Michael Boeckeler, Irina Bonzheim, Florian Schimmele, Werner Spengler, Franz Stanzel, Christoph Petermann, Kaid Darwiche , Lars Hagmeyer, Reinhard Buettner, Markus Tiemann, Hans-Ulrich Schildhaus, Rainer Muche, Hans Boesmueller, Felix Everinghoff, Robert Mueller, Bijoy Atique, Richard A. Lewis, Lars Zender, Falko Fend and Juergen Hetzel
The detection of molecular alterations is crucial for the individualized treatment of advanced non-small cell lung cancer (NSCLC). Missing targetable alterations may have a major impact on patient’s progression free and overall survival. Although laboratory testing for molecular alterations has continued to improve; little is known about how biopsy technique affects the detection rate of different mutations. In the retrospective study detection rate of epidermal growth factor (EGFR) mutations in tissue extracted by bronchoscopic cryobiopsy (CB was significantly higher compared to other standard biopsy techniques. This prospective, randomized, multicenter, single blinded study evaluates the accuracy of molecular genetic characterization of NSCLC for different cell sampling techniques. Key inclusion criteria are suspected lung cancer or the suspected relapse of known NSCLC that is bronchoscopically visible. Patients will be randomized, either to have a CB or a bronchoscopic forceps biopsy (FB). If indicated, a transbronchial needle aspiration (TBNA) of suspect lymph nodes will be performed. Blood liquid biopsy will be taken before tissue biopsy. The primary endpoint is the detection rate of molecular genetic alterations in NSCLC, using CB and FB. Secondary endpoints are differences in the combined detection of molecular genetic alterations between FB and CB, TBNA and liquid biopsy. This trial plans to recruit 540 patients, with 178 evaluable patients per study cohort. A histopathological and molecular genetic evaluation will be performed by the affiliated pathology departments of the national network for genomic medicine in lung cancer (nNGM), Germany. We will compare the diagnostic value of solid tumor tissue, lymph node cells and liquid biopsy for the molecular genetic characterization of NSCLC. This reflects a real world clinical setting, with potential direct impact on both treatment and survival.
Stefanie Schatz*, Markus Falk*, Balázs Jóri*, Hayat O Ramdani, Stefanie Schmidt, Eva-Maria Willing, Roopika Menon, Harry J M Groen, Linda Diehl, Matthias Kröger, Claas Wesseler, Frank Griesinger, Petra Hoffknecht, Markus Tiemann, Lukas C Heukamp
Cancers (Basel). 2020 Jun 24;12(6):E1685. doi: 10.3390/cancers12061685
PMID: 32599951
Atiakshin D, Buchwalow I, Tiemann M.
Histochem Cell Biol. 2020 Mar 28. doi: 10.1007/s00418-020-01875-9.
PMID: 32222902
Klenke S, Akdeli N, Stelmach P, Heukamp L, Schulte JH, Bachmann HS.
BMC Cancer. 2019 Mar 18;19(1):243. doi: 10.1186/s12885-019-5439-1.
PMID: 30885150
Gergs U, Bernhardt G, Buchwalow IB, Edler H, Fröba J, Keller M, Kirchhefer U, Köhler F, Mißlinger N, Wache H, Neumann J.
J Pharmacol Exp Ther. 2019 Apr;369(1):129-141. doi: 10.1124/jpet.118.255711. Epub 2019 Feb 6.
PMID: 30728249
Boecker W, Reusch M, Mielke V, Reusch U, Loening T, Tiemann M, Buchwalow I.
J Dermatol. 2019 Jan 21. doi: 10.1111/1346-8138.14765. [Epub ahead of print]
PMID: 30663115
Buchwalow I, Atjakshin D, Pawlowa T, Tiemann M
„Histochemistry: Textbook“ Woronesch: Isdatelstvo-Poligrafitscheski Zenter „Nautschnaja Kniga“, 2018. — 226 с. ISBN 978-5-4446-1036-7
Buchwalow I, Schnekenburger J, Samoilova V, Boecker W, Neumann J, and Tiemann K.
Acta Histochem. Cytochem. 2018 Nov; 51 (5): DOI: 10.1267/ahc.18028.
Buchwalow I, Schnekenburger J, Samoilova V, Boecker W, Neumann J, Tiemann K. Acta Histochem Cytochem, 2018 Dec 20;51 (6):167-172. Doi: 10.1267/ahc.18028.Epub 2018 Nov 3. PMID: 30647491
Tiemann K, Schnekenburger J, Schick V, Demus U, Müller-Werdan U, Atiakshin D, Boecker W, Samoilova V, Buchwalow I.
Biomed. J. Sci. Tech. Res. 2018 Oct; 4:1-6.MS.ID.001063. DOI: 10.26717/BJSTR.2018.04.001063
Tolksdorf F, Mikulec J, Geers B, Endig J, Sprezyna P, Heukamp LC, Knolle PA, Kolanus W, Diehl L.
Sci Rep. 2018 Oct 31;8(1):16123. doi: 10.1038/s41598-018-34522-4.
PMID: 30382149
Hallas C, Preukschas M, Tiemann M.
Leuk Res. 2019 Jan;76:107-111. doi: 10.1016/j.leukres.2018.10.003. Epub 2018 Oct 10.
PMID: 30360939
Berger LA, Janning M, Velthaus JL, Ben-Batalla I, Schatz S, Falk M, Iglauer P, Simon R, Cao R, Forcato C, Manaresi N, Bramlett K, Buson G, Hanssen A, Tiemann M, Sauter G, Bokemeyer C, Riethdorf S, Reck M, Pantel K, Wikman H, Loges S.
J Thorac Oncol. 2018 Dec;13(12):e243-e246. doi: 10.1016/j.jtho.2018.08.2025. Epub 2018 Sep 8. No abstract available.
PMID: 30205165
W. Boecker, L. van Horn, G. Stenman, C. Sturken, U. Schumacher, T. Loening, L. Liesenfeld, E. Korsching, D. Glaser, K. Tiemann, I. Buchwalow
Lab Invest, (2018) Volume 98 (8) 65-1075
Bruckl, W. M.; Ficker, J. H.; Tiemann, M.; Schumann, C.; Reinmuth, N.; Heigener, D.
Schutte, W.; Eberhardt, R.; Darwiche, K.; Wagner, M.
Pneumologie. 2018 Sep; 72(9):617-623. doi: 10.1055/a-0632-9174. [Epub 2018 Aug 2.]
PMID: 30071539
Frank R, Scheffler M, Merkelbach-Bruse S, Ihle MA, Kron A, Rauer M, Ueckeroth F, König K, Michels S, Fischer R, Eisert A, Fassunke J, Heydt C, Serke M, Ko YD, Gerigk U, Geist T, Kaminsky B, Heukamp LC, Clement-Ziza M, Büttner R, Wolf J.
Clin Cancer Res. 2018 Jul 1;24(13):3087-3096. doi: 10.1158/1078-0432.CCR-17-3416. Epub 2018 Apr 3.
PMID: 29615460
Krause T, Grote-Wessels S, Balzer F, Boknik P, Gergs U, Kirchhefer U, Buchwalow IB, Müller FU, Schmitz W, Neumann J.
Naunyn Schmiedebergs Arch Pharmacol. 2018 Aug;391(8):859-873. doi: 10.1007/s00210-018-1515-3. Epub 2018 May 24.
PMID: 29797049
Buchwalow I, Samoilova V, Boecker W, Tiemann M.
Acta Histochem. 2018 May 5. pii: S0065-1281(18)30039-4. doi: 10.1016/j.acthis.2018.05.002.
PMID: 29739626
Keppens C, Palma JF, Das PM, Scudder S, Wen W, Normanno N, van Krieken JH, Sacco A, Fenizia F, Gonzalez de Castro D, Hönigschnabl S, Kern I, Lopez-Rios F, Lozano MD, Marchetti A, Halfon P, Schuuring E, Setinek U, Sorensen B, Taniere P, Tiemann M, Vosmikova H, Dequeker EMC.
J Mol Diagn. 2018 Jul;20(4):483-494. doi: 10.1016/j.jmoldx.2018.03.006. Epub 2018 Apr 26.
PMID: 29704571
Atiakshin D, Buchwalow I, Samoilova V, Tiemann M.
Histochem Cell Biol. 2018 Mar 12. doi: 10.1007/s00418-018-1659-8.
PMID: 29532158
Meder L, König K, Dietlein F, Macheleidt I, Florin A, Ercanoglu MS, Rommerscheidt-Fuss U, Koker M, Schön G, Odenthal M, Klein F, Büttner R, Schulte JH, Heukamp LC, Ullrich RT.
Oncogene. 2018 May;37(20):2746-2756. doi: 10.1038/s41388-018-0158-7. Epub 2018 Mar 5.
PMID: 29503447
Mahjoubi L, Cecchi F, Massard C, Calabro F, Gazzah A, Bahleda R, Jamme P, Gelli M, Goere D, Lacroix L, Adam J, Heukamp L, Trenta P, Hembrough T, Soria JC, Sternberg C, Ducreux M.
Tumori. 2018 Mar 1:300891618763215. doi: 10.1177/0300891618763215. [Epub ahead of print]
PMID: 29714649
Buchwalow I, Atiakshin D, Samoilova V, Boecker W, Tiemann M.
Histochem Cell Biol. 2018 Feb;149(2):169-177. doi: 10.1007/s00418-017-1624-y. Epub 2017 Dec 2.
PMID: 29197996
Scheel AH, Baenfer G, Baretton G, Dietel M, Diezko R, Henkel T, Heukamp LC, Jasani B, Jöhrens K, Kirchner T, Lasitschka F, Petersen I, Reu S, Schildhaus HU, Schirmacher P, Schwamborn K, Sommer U, Stoss O, Tiemann M, Warth A, Weichert W, Wolf J, Büttner R, Rüschoff J.
Histopathology. 2018 Feb;72(3):449-459. doi: 10.1111/his.13375. Epub 2017 Nov 21.
PMID: 28851100
Bailey DL, Pichler BJ, Gückel B, Antoch G, Barthel H, Bhujwalla ZM, Biskup S, Biswal S, Bitzer M, Boellaard R, Braren RF, Brendle C, Brindle K, Chiti A, la Fougère C, Gillies R, Goh V, Goyen M, Hacker M, Heukamp L, Knudsen GM, Krackhardt AM, Law I, Morris JC, Nikolaou K, Nuyts J, Ordonez AA, Pantel K, Quick HH, Riklund K, Sabri O, Sattler B, Troost EGC, Zaiss M, Zender L, Beyer T.
Mol Imaging Biol. 2018 Feb;20(1):4-20. doi: 10.1007/s11307-017-1123-5.
PMID: 28971346
Plenker D, Bertrand M, de Langen AJ, Riedel R, Lorenz C, Scheel AH, Müller J, Brägelmann J, Daßler-Plenker J, Kobe C, Persigehl T, Kluge A, Wurdinger T, Schellen P, Hartmann G, Zacherle T, Menon R, Thunnissen E, Büttner R, Griesinger F, Wolf J, Heukamp L, Sos ML, Heuckmann JM.
Clin Cancer Res. 2018 Mar 15;24(6):1337-1343. doi: 10.1158/1078-0432.CCR-17-3001. Epub 2017 Dec 28.
PMID: 29284707
Petersen I, Dietel M, Geilenkeuser WJ, Mireskandari M, Weichert W, Steiger K, Scheel AH, Büttner R, Schirmacher P, Warth A, Lasitschka F, Schildhaus HU, Kirchner T, Reu S, Kreipe H, Länger F, Tiemann M, Schulte C, Jöhrens K.
Pathol Res Pract. 2017 Dec;213(12):1530-1535. doi: 10.1016/j.prp.2017.09.021. Epub 2017 Sep 28.
PMID: 29108919
Müller JN, Falk M, Talwar J, Neemann N, Mariotti E, Bertrand M, Zacherle T, Lakis S, Menon R, Gloeckner C, Tiemann M, Heukamp LC, Thomas RK, Griesinger F, Heuckmann JM.
J Thorac Oncol. 2017 Oct;12(10):1503-1511. doi: 10.1016/j.jtho.2017.07.014. Epub 2017 Jul 24.
PMID: 28751246
Brägelmann J, Dammert MA, Dietlein F, Heuckmann JM, Choidas A, Böhm S, Richters A, Basu D, Tischler V, Lorenz C, Habenberger P, Fang Z, Ortiz-Cuaran S, Leenders F, Eickhoff J, Koch U, Getlik M, Termathe M, Sallouh M, Greff Z, Varga Z, Balke-Want H, French CA, Peifer M, Reinhardt HC, Örfi L, Kéri G, Ansén S, Heukamp LC, Büttner R, Rauh D, Klebl BM, Thomas RK, Sos ML.
Cell Rep. 2017 Sep 19;20(12):2833-2845. doi: 10.1016/j.celrep.2017.08.082.
PMID: 28930680
Lakis S, Heukamp LC, Griesinger F.
J Thorac Oncol. 2017 Nov;12(11):e186-e188. doi: 10.1016/j.jtho.2017.07.018. No abstract available.
PMID: 29074212
Atiakshin D, Samoilova V, Buchwalow I, Boecker W, Tiemann M.
Histochem Cell Biol. 2017 Jun;147(6):683-694. doi: 10.1007/s00418-017-1547-7. Epub 2017 Feb 27.
PMID: 28243739
Boecker W, Stenman G, Schroeder T, Schumacher U, Loening T, Stahnke L, Löhnert C, Siering RM, Kuper A, Samoilova V, Tiemann M, Korsching E, Buchwalow I.
Virchows Arch. 2017 May;470(5):493-504. doi: 10.1007/s00428-017-2073-7. Epub 2017 Mar 16.
PMID: 28303349
Gottlieb A, Althoff K, Grunewald L, Thor T, Odersky A, Schulte M, Deubzer HE, Heukamp L, Eggert A, Schramm A, Schulte JH, Künkele A.
Oncotarget. 2017 Apr 25;8(17):27882-27891. doi: 10.18632/oncotarget.15840.
PMID: 28427187
Ladang A, Rapino F, Heukamp LC, Tharun L, Shostak K, Hermand D, Delaunay S, Klevernic I, Jiang Z, Jacques N, Jamart D, Migeot V, Florin A, Göktuna S, Malgrange B, Sansom OJ, Nguyen L, Büttner R, Close P, Chariot A.
J Exp Med. 2017 Apr 3;214(4):1199. doi: 10.1084/jem.2014228803082017c. Epub 2017 Mar 10. No abstract available.
PMID: 28283535
Michels SYF, Scheel AH, Wündisch T, Heuckmann JM, Menon R, Puesken M, Kobe C, Pasternack H, Heydt C, Scheffler M, Fischer R, Schultheis AM, Merkelbach-Bruse S, Heukamp L, Büttner R, Wolf J.
NPJ Precis Oncol. 2017 Mar 20;1(1):4. doi: 10.1038/s41698-017-0004-3. eCollection 2017.
PMID: 29872693
Atiakshin D, Samoilova V, Buchwalow I, Boecker W, Tiemann M.
Histochem Cell Biol. 2017 Jun;147(6):683-694. doi: 10.1007/s00418-017-1547-7. Epub 2017 Feb 27.
PMID: 28243739
Buchwalow I, Schnekenburger J, Atiakshin D, Samoilova V, Wolf E, Boecker W, Tiemann K.
Acta Histochem. 2017 Apr;119(3):252-256. doi: 10.1016/j.acthis.2017.01.010. Epub 2017 Feb 3.
PMID: 28168994
Locati LD, Collini P, Imbimbo M, Barisella M, Testi A, Licitra LF, Löning T, Tiemann K, Quattrone P, Bimbatti E, Chiaravalli S, Casanova M, Tamborini E, Carta R, Gasparini P, Guzzo M, Massimino M, Ferrari A.
Pediatr Blood Cancer. 2017 Jan 31. doi: 10.1002/pbc.26468. [Epub ahead of print]
PMID: 28139061
Pajtler KW, Sadowski N, Ackermann S, Althoff K, Schönbeck K, Batzke K, Schäfers S, Odersky A, Heukamp L, Astrahantseff K, Künkele A, Deubzer HE, Schramm A, Sprüssel A, Thor T, Lindner S, Eggert A, Fischer M, Schulte JH.
Oncotarget. 2017 Jan 24;8(4):6730-6741. doi: 10.18632/oncotarget.14268.
PMID: 28036269
Scheel AH, Dietel M, Heukamp LC, Jöhrens K, Kirchner T, Reu S, Rüschoff J, Schildhaus HU, Schirmacher P, Tiemann M, Warth A, Weichert W, Fischer RN, Wolf J, Büttner R.
Pathologe. 2016 Nov;37(6):557-567. Review. German.
PMID: 27510417
Scheel AH, Dietel M, Heukamp LC, Jöhrens K, Kirchner T, Reu S, Rüschoff J, Schildhaus HU, Schirmacher P, Tiemann M, Warth A, Weichert W, Fischer RN, Wolf J, Buettner R.
Mod Pathol. 2016 Oct;29(10):1165-72. doi: 10.1038/modpathol.2016.117. Epub 2016 Jul 8.
PMID: 27389313
Hallas C, Phillipp J, Domanowsky L, Kah B, Tiemann K.
BMC Cancer. 2016 Aug 18;16:648. doi: 10.1186/s12885-016-2707-1.
PMID: 27539223
Scheel AH, Dietel M, Heukamp LC, Jöhrens K, Kirchner T, Reu S, Rüschoff J, Schildhaus HU, Schirmacher P, Tiemann M, Warth A, Weichert W, Fischer RN, Wolf J, Buettner R.
Mod Pathol. 2016 Oct;29(10):1165-72. doi: 10.1038/modpathol.2016.117. Epub 2016 Jul 8.
PMID: 27389313
Loibl S, Darb-Esfahani S, Huober J, Klimowicz A, Furlanetto J, Lederer B, Hartmann A, Eidtmann H, Pfitzner B, Fasching PA, Tiemann K, Jackisch C, Mehta K, von Minckwitz G, Untch M, Denkert C.
Clin Cancer Res. 2016 Jun 1;22(11):2675-83. doi: 10.1158/1078-0432.CCR-15-0965. Epub 2016 Jan 12.
PMID: 26758558
Buchwalow I, Boecker W, Tiemann M.
Virchows Arch. 2015 Jan;466(1):111-6. doi: 10.1007/s00428-014-1677-4. Epub 2014 Oct 31.
PMID: 25359602
Buchwalow I, Boecker W, Tiemann M.
Virchows Arch. 2015 Jan;466(1):111-6. doi: 10.1007/s00428-014-1677-4. Epub 2014 Oct 31.
PMID: 25359602
Boecker W, Stenman G, Loening T, Andersson MK, Berg T, Lange A, Bankfalvi A, Samoilova V, Tiemann K, Buchwalow I.
Virchows Arch. 2015 Jan;466(1):21-36. doi: 10.1007/s00428-014-1671-x. Epub 2014 Oct 26.
PMID: 25344874
Maass N, Schem C, Bauerschlag DO, Tiemann K, Schaefer FW, Hanson S, Muth M, Baier M, Weigel MT, Wenners AS, Alkatout I, Bauer M, Jonat W, Mundhenke C.
Oncology. 2014;87(5):300-10. doi: 10.1159/000365553. Epub 2014 Aug 26.
PMID: 25171229
Tiemann M, Hallas C, Falk M.
Trillium Krebsmedizin 2014, 23:6
Tiemann K, Weigel MT, Alkatout I, Wenners AS, Mundhenke H, Schäfer FW, Bauer M, Schem C, Maass N, Jonat W, Mundhenke C.
Anticancer Res. 2014 Jul;34(7):3607-16.
PMID: 24982376
W. Boecker, G. Stenman, T. Loening, M.K. Andersson, H.P. Sinn, P. Barth, F. Oberhellmann, I. Bos, T. Berg, Z. Marusic, V. Samoilova, I. Buchwalow
Histopathology, 65 (2014) 9-23
Buchwalow I, Boecker W, Wolf E, Samoilova V, Tiemann M.
Acta Histochem. 2013 Jul;115(6):587-94. doi: 10.1016/j.acthis.2012.12.008. Epub 2013 Jan 23.
PMID: 23351717
Buchwalow I, Schnekenburger J, Tiemann K, Samoilova V, Bankfalvi A, Poremba C, Schleicher C, Neumann J, Boecker W.
Sci Rep. 2013 May 28;3:1899. doi: 10.1038/srep01899.
PMID: 23712581
Boecker W, Stenman G, Loening T, Andersson MK, Bankfalvi A, von Holstein S, Heegaard S, Lange A, Berg T, Samoilova V, Tiemann K, Buchwalow I.
Mod Pathol. 2013 Apr 5. doi: 10.1038/modpathol.2013.45. [Epub ahead of print]
PMID: 23558567
Buchwalow I, Boecker W, Wolf E, Samoilova V, Tiemann M.
Acta Histochem. 2013 Jan 23. doi:pii: S0065-1281(12)00183-3. 10.1016/j.acthis. 2012.12.008.
PMID: 23351717
Die „stille Revolution“ in der Behandlung solider Tumoren. Falk M, Hallas C, Tiemann M.
best practice onkologie 2012 February 1:4
DOI: 10.1007/s11654-011-0335-4. ISSN: 0946-4565.
Boecker W, Junkers T, Reusch M, Buerger H, Korsching E, Metze D, Decker T, Loening T, Lange A, Samoilova V, Buchwalow I.
Sci Rep. 2012;2:226. doi: 10.1038/srep00226. Epub 2012 Jan 17. No abstract available.
PMID: 22355740
Buchwalow I, Samoilova V, Boecker W, Tiemann M.
Sci Rep. 2011;1:28. doi: 10.1038/srep00028. Epub 2011 Jul 1.
PMID: 22355547
I.B. Buchwalow, W. Boecker
1 ed., Springer, Heidelberg, Dordrecht, London, New York, 2010
I.B. Buchwalow, S. Cacanyiova, J. Neumann, V.E. Samoilova, W. Boecker, F. Kristek
Biochem Biophys Res Commun, 377 (2008) 504-507