Influence of Biopsy Technique on Molecular Genetic Tumor Characterization in Non-Small Cell Lung Cancer—The Prospective, Randomized, Single-Blinded, Multicenter PROFILER Study Protocol

Maik Haentschel*, Michael Boeckeler, Irina Bonzheim, Florian Schimmele, Werner Spengler, Franz Stanzel, Christoph Petermann, Kaid Darwiche , Lars Hagmeyer, Reinhard Buettner, Markus Tiemann, Hans-Ulrich Schildhaus, Rainer Muche, Hans Boesmueller, Felix Everinghoff, Robert Mueller, Bijoy Atique, Richard A. Lewis, Lars Zender, Falko Fend and Juergen Hetzel

The detection of molecular alterations is crucial for the individualized treatment of advanced non-small cell lung cancer (NSCLC). Missing targetable alterations may have a major impact on patient’s progression free and overall survival. Although laboratory testing for molecular alterations has continued to improve; little is known about how biopsy technique affects the detection rate of different mutations. In the retrospective study detection rate of epidermal growth factor (EGFR) mutations in tissue extracted by bronchoscopic cryobiopsy (CB was significantly higher compared to other standard biopsy techniques. This prospective, randomized, multicenter, single blinded study evaluates the accuracy of molecular genetic characterization of NSCLC for different cell sampling techniques. Key inclusion criteria are suspected lung cancer or the suspected relapse of known NSCLC that is bronchoscopically visible. Patients will be randomized, either to have a CB or a bronchoscopic forceps biopsy (FB). If indicated, a transbronchial needle aspiration (TBNA) of suspect lymph nodes will be performed. Blood liquid biopsy will be taken before tissue biopsy. The primary endpoint is the detection rate of molecular genetic alterations in NSCLC, using CB and FB. Secondary endpoints are differences in the combined detection of molecular genetic alterations between FB and CB, TBNA and liquid biopsy. This trial plans to recruit 540 patients, with 178 evaluable patients per study cohort. A histopathological and molecular genetic evaluation will be performed by the affiliated pathology departments of the national network for genomic medicine in lung cancer (nNGM), Germany. We will compare the diagnostic value of solid tumor tissue, lymph node cells and liquid biopsy for the molecular genetic characterization of NSCLC. This reflects a real world clinical setting, with potential direct impact on both treatment and survival.

Integration of Tumor Mutation Burden and PD-L1 Testing in Routine Laboratory Diagnostics in Non-Small Cell Lung Cancer

Stefanie Schatz*, Markus Falk*, Balázs Jóri*, Hayat O Ramdani, Stefanie Schmidt, Eva-Maria Willing, Roopika Menon, Harry J M Groen, Linda Diehl, Matthias Kröger, Claas Wesseler, Frank Griesinger, Petra Hoffknecht, Markus Tiemann, Lukas C Heukamp
Cancers (Basel). 2020 Jun 24;12(6):E1685. doi: 10.3390/cancers12061685
PMID: 32599951

Mast cells and collagen fibrillogenesis

Atiakshin D, Buchwalow I, Tiemann M.
Histochem Cell Biol. 2020 Mar 28. doi: 10.1007/s00418-020-01875-9.
PMID: 32222902

The small molecule Bcl-2/Mcl-1 inhibitor TW-37 shows single-agent cytotoxicity in neuroblastoma cell lines

Klenke S, Akdeli N, Stelmach P, Heukamp L, Schulte JH, Bachmann HS.
BMC Cancer. 2019 Mar 18;19(1):243. doi: 10.1186/s12885-019-5439-1.
PMID: 30885150

Initial Characterization of Transgenic Mice Overexpressing Human Histamine H2 Receptors

Gergs U, Bernhardt G, Buchwalow IB, Edler H, Fröba J, Keller M, Kirchhefer U, Köhler F, Mißlinger N, Wache H, Neumann J.
J Pharmacol Exp Ther. 2019 Apr;369(1):129-141. doi: 10.1124/jpet.118.255711. Epub 2019 Feb 6.
PMID: 30728249

Spatial analysis of p63, K5 and K7 defines two groups of progenitor cells that differentially contribute to the maintenance of normal sebaceous glands, extraocular sebaceous carcinoma and benign sebaceous tumors

Boecker W, Reusch M, Mielke V, Reusch U, Loening T, Tiemann M, Buchwalow I.
J Dermatol. 2019 Jan 21. doi: 10.1111/1346-8138.14765. [Epub ahead of print]
PMID: 30663115

Histochimija: Metoditscheskoje Possobije

Buchwalow I, Atjakshin D, Pawlowa T, Tiemann M
„Histochemistry: Textbook“ Woronesch: Isdatelstvo-Poligrafitscheski Zenter „Nautschnaja Kniga“, 2018. — 226 с. ISBN 978-5-4446-1036-7

New insight into the role of nitric oxide pathways in pancreas

Buchwalow I, Schnekenburger J, Samoilova V, Boecker W, Neumann J, and Tiemann K.
Acta Histochem. Cytochem. 2018 Nov; 51 (5): DOI: 10.1267/ahc.18028.

New Insight into the Role of Nitric Oxide Pathways in Pancreas

Buchwalow I, Schnekenburger J, Samoilova V, Boecker W, Neumann J, Tiemann K. Acta Histochem Cytochem, 2018 Dec 20;51 (6):167-172. Doi: 10.1267/ahc.18028.Epub 2018 Nov 3. PMID: 30647491

Oxidative stress and NO generation in cerulein-induced rat pancreatitis

Tiemann K, Schnekenburger J, Schick V, Demus U, Müller-Werdan U, Atiakshin D, Boecker W, Samoilova V, Buchwalow I.
Biomed. J. Sci. Tech. Res. 2018 Oct; 4:1-6.MS.ID.001063. DOI: 10.26717/BJSTR.2018.04.001063

The PDL1-inducible GTPase Arl4d controls T effector function by limiting IL-2 production

Tolksdorf F, Mikulec J, Geers B, Endig J, Sprezyna P, Heukamp LC, Knolle PA, Kolanus W, Diehl L.
Sci Rep. 2018 Oct 31;8(1):16123. doi: 10.1038/s41598-018-34522-4.
PMID: 30382149

Immunohistochemical distinction of ABC and GCB in extranodal DLBCL is not reflected in mutation patterns

Hallas C, Preukschas M, Tiemann M.
Leuk Res. 2019 Jan;76:107-111. doi: 10.1016/j.leukres.2018.10.003. Epub 2018 Oct 10.
PMID: 30360939

Identification of a High-Level MET Amplification in CTCs and cfTNA of an ALK-Positive NSCLC Patient Developing Evasive Resistance to Crizotinib

Berger LA, Janning M, Velthaus JL, Ben-Batalla I, Schatz S, Falk M, Iglauer P, Simon R, Cao R, Forcato C, Manaresi N, Bramlett K, Buson G, Hanssen A, Tiemann M, Sauter G, Bokemeyer C, Riethdorf S, Reck M, Pantel K, Wikman H, Loges S.
J Thorac Oncol. 2018 Dec;13(12):e243-e246. doi: 10.1016/j.jtho.2018.08.2025. Epub 2018 Sep 8. No abstract available.
PMID: 30205165

Spatially correlated phenotyping reveals K5-positive luminal progenitor cells and p63-K5/14-positive stem cell-like cells in human breast epithelium

W. Boecker, L. van Horn, G. Stenman, C. Sturken, U. Schumacher, T. Loening, L. Liesenfeld, E. Korsching, D. Glaser, K. Tiemann, I. Buchwalow
Lab Invest, (2018) Volume 98 (8) 65-1075

[Rebiopsy for Patients with Lung Cancer – Joint Opinion from both the Endoscopic and Thoracic Oncology Sections of the German Society of Pneumology (DGP)]

Bruckl, W. M.; Ficker, J. H.; Tiemann, M.; Schumann, C.; Reinmuth, N.; Heigener, D.
Schutte, W.; Eberhardt, R.; Darwiche, K.; Wagner, M.
Pneumologie. 2018 Sep; 72(9):617-623. doi: 10.1055/a-0632-9174. [Epub 2018 Aug 2.]
PMID: 30071539

Clinical and Pathological Characteristics of KEAP1- and NFE2L2-Mutated Non-Small Cell Lung Carcinoma (NSCLC)

Frank R, Scheffler M, Merkelbach-Bruse S, Ihle MA, Kron A, Rauer M, Ueckeroth F, König K, Michels S, Fischer R, Eisert A, Fassunke J, Heydt C, Serke M, Ko YD, Gerigk U, Geist T, Kaminsky B, Heukamp LC, Clement-Ziza M, Büttner R, Wolf J.
Clin Cancer Res. 2018 Jul 1;24(13):3087-3096. doi: 10.1158/1078-0432.CCR-17-3416. Epub 2018 Apr 3.
PMID: 29615460

Successful overexpression of wild-type inhibitor-2 of PP1 in cardiovascular cells

Krause T, Grote-Wessels S, Balzer F, Boknik P, Gergs U, Kirchhefer U, Buchwalow IB, Müller FU, Schmitz W, Neumann J.
Naunyn Schmiedebergs Arch Pharmacol. 2018 Aug;391(8):859-873. doi: 10.1007/s00210-018-1515-3. Epub 2018 May 24.
PMID: 29797049

Multiple immunolabeling with antibodies from the same host species in combination with tyramide signal amplification.

Buchwalow I, Samoilova V, Boecker W, Tiemann M.
Acta Histochem. 2018 May 5. pii: S0065-1281(18)30039-4. doi: 10.1016/j.acthis.2018.05.002.
PMID: 29739626

Detection of EGFR Variants in Plasma: A Multilaboratory Comparison of a Real-Time PCR EGFR Mutation Test in Europe

Keppens C, Palma JF, Das PM, Scudder S, Wen W, Normanno N, van Krieken JH, Sacco A, Fenizia F, Gonzalez de Castro D, Hönigschnabl S, Kern I, Lopez-Rios F, Lozano MD, Marchetti A, Halfon P, Schuuring E, Setinek U, Sorensen B, Taniere P, Tiemann M, Vosmikova H, Dequeker EMC.
J Mol Diagn. 2018 Jul;20(4):483-494. doi: 10.1016/j.jmoldx.2018.03.006. Epub 2018 Apr 26.
PMID: 29704571

Tryptase as a polyfunctional component of mast cells.

Atiakshin D, Buchwalow I, Samoilova V, Tiemann M.
Histochem Cell Biol. 2018 Mar 12. doi: 10.1007/s00418-018-1659-8.
PMID: 29532158

LIN28B enhanced tumorigenesis in an autochthonous KRASG12V-driven lung carcinoma mouse model

Meder L, König K, Dietlein F, Macheleidt I, Florin A, Ercanoglu MS, Rommerscheidt-Fuss U, Koker M, Schön G, Odenthal M, Klein F, Büttner R, Schulte JH, Heukamp LC, Ullrich RT.
Oncogene. 2018 May;37(20):2746-2756. doi: 10.1038/s41388-018-0158-7. Epub 2018 Mar 5.
PMID: 29503447

Personalized therapy based on sequential molecular analysis leads to 30 months of survival in a patient with diffuse unresectable gastric linitis plastica

Mahjoubi L, Cecchi F, Massard C, Calabro F, Gazzah A, Bahleda R, Jamme P, Gelli M, Goere D, Lacroix L, Adam J, Heukamp L, Trenta P, Hembrough T, Soria JC, Sternberg C, Ducreux M.
Tumori. 2018 Mar 1:300891618763215. doi: 10.1177/0300891618763215. [Epub ahead of print]
PMID: 29714649

Identification of autofluorescent cells in human angioimmunoblastic T-cell lymphoma.

Buchwalow I, Atiakshin D, Samoilova V, Boecker W, Tiemann M.
Histochem Cell Biol. 2018 Feb;149(2):169-177. doi: 10.1007/s00418-017-1624-y. Epub 2017 Dec 2.
PMID: 29197996

Interlaboratory concordance of PD-L1 immunohistochemistry for non-small-cell lung cancer.

Scheel AH, Baenfer G, Baretton G, Dietel M, Diezko R, Henkel T, Heukamp LC, Jasani B, Jöhrens K, Kirchner T, Lasitschka F, Petersen I, Reu S, Schildhaus HU, Schirmacher P, Schwamborn K, Sommer U, Stoss O, Tiemann M, Warth A, Weichert W, Wolf J, Büttner R, Rüschoff J.
Histopathology. 2018 Feb;72(3):449-459. doi: 10.1111/his.13375. Epub 2017 Nov 21.
PMID: 28851100

Combined PET/MRI: Global Warming-Summary Report of the 6th International Workshop on PET/MRI, March 27-29, 2017, Tübingen, Germany

Bailey DL, Pichler BJ, Gückel B, Antoch G, Barthel H, Bhujwalla ZM, Biskup S, Biswal S, Bitzer M, Boellaard R, Braren RF, Brendle C, Brindle K, Chiti A, la Fougère C, Gillies R, Goh V, Goyen M, Hacker M, Heukamp L, Knudsen GM, Krackhardt AM, Law I, Morris JC, Nikolaou K, Nuyts J, Ordonez AA, Pantel K, Quick HH, Riklund K, Sabri O, Sattler B, Troost EGC, Zaiss M, Zender L, Beyer T.
Mol Imaging Biol. 2018 Feb;20(1):4-20. doi: 10.1007/s11307-017-1123-5.
PMID: 28971346

Structural Alterations of MET Trigger Response to MET Kinase Inhibition in Lung Adenocarcinoma Patients

Plenker D, Bertrand M, de Langen AJ, Riedel R, Lorenz C, Scheel AH, Müller J, Brägelmann J, Daßler-Plenker J, Kobe C, Persigehl T, Kluge A, Wurdinger T, Schellen P, Hartmann G, Zacherle T, Menon R, Thunnissen E, Büttner R, Griesinger F, Wolf J, Heukamp L, Sos ML, Heuckmann JM.
Clin Cancer Res. 2018 Mar 15;24(6):1337-1343. doi: 10.1158/1078-0432.CCR-17-3001. Epub 2017 Dec 28.
PMID: 29284707

EGFR immunohistochemistry as biomarker for antibody-based therapy of squamous NSCLC – Experience from the first ring trial of the German Quality Assurance Initiative for Pathology (QuIP®).

Petersen I, Dietel M, Geilenkeuser WJ, Mireskandari M, Weichert W, Steiger K, Scheel AH, Büttner R, Schirmacher P, Warth A, Lasitschka F, Schildhaus HU, Kirchner T, Reu S, Kreipe H, Länger F, Tiemann M, Schulte C, Jöhrens K.
Pathol Res Pract. 2017 Dec;213(12):1530-1535. doi: 10.1016/j.prp.2017.09.021. Epub 2017 Sep 28.
PMID: 29108919

Concordance between Comprehensive Cancer Genome Profiling in Plasma and Tumor Specimens.

Müller JN, Falk M, Talwar J, Neemann N, Mariotti E, Bertrand M, Zacherle T, Lakis S, Menon R, Gloeckner C, Tiemann M, Heukamp LC, Thomas RK, Griesinger F, Heuckmann JM.
J Thorac Oncol. 2017 Oct;12(10):1503-1511. doi: 10.1016/j.jtho.2017.07.014. Epub 2017 Jul 24.
PMID: 28751246

Systematic Kinase Inhibitor Profiling Identifies CDK9 as a Synthetic Lethal Target in NUT Midline Carcinoma

Brägelmann J, Dammert MA, Dietlein F, Heuckmann JM, Choidas A, Böhm S, Richters A, Basu D, Tischler V, Lorenz C, Habenberger P, Fang Z, Ortiz-Cuaran S, Leenders F, Eickhoff J, Koch U, Getlik M, Termathe M, Sallouh M, Greff Z, Varga Z, Balke-Want H, French CA, Peifer M, Reinhardt HC, Örfi L, Kéri G, Ansén S, Heukamp LC, Büttner R, Rauh D, Klebl BM, Thomas RK, Sos ML.
Cell Rep. 2017 Sep 19;20(12):2833-2845. doi: 10.1016/j.celrep.2017.08.082.
PMID: 28930680

Detection of Discrepant Driver Mutations in a Patient with Two Synchronous Primary Non-Small Cell Lung Cancers (NSCLCs) with Liquid Biopsy

Lakis S, Heukamp LC, Griesinger F.
J Thorac Oncol. 2017 Nov;12(11):e186-e188. doi: 10.1016/j.jtho.2017.07.018. No abstract available.
PMID: 29074212

Characterization of mast cell populations using different methods for their identification.

Atiakshin D, Samoilova V, Buchwalow I, Boecker W, Tiemann M.
Histochem Cell Biol. 2017 Jun;147(6):683-694. doi: 10.1007/s00418-017-1547-7. Epub 2017 Feb 27.
PMID: 28243739

Multicolor immunofluorescence reveals that p63- and/or K5-positive progenitor cells contribute to normal breast epithelium and usual ductal hyperplasia but not to low-grade intraepithelial neoplasia of the breast.

Boecker W, Stenman G, Schroeder T, Schumacher U, Loening T, Stahnke L, Löhnert C, Siering RM, Kuper A, Samoilova V, Tiemann M, Korsching E, Buchwalow I.
Virchows Arch. 2017 May;470(5):493-504. doi: 10.1007/s00428-017-2073-7. Epub 2017 Mar 16.
PMID: 28303349

RITA displays anti-tumor activity in medulloblastomas independent of TP53 status

Gottlieb A, Althoff K, Grunewald L, Thor T, Odersky A, Schulte M, Deubzer HE, Heukamp L, Eggert A, Schramm A, Schulte JH, Künkele A.
Oncotarget. 2017 Apr 25;8(17):27882-27891. doi: 10.18632/oncotarget.15840.
PMID: 28427187

Correction: Elp3 drives Wnt-dependent tumor initiation and regeneration in the intestine

Ladang A, Rapino F, Heukamp LC, Tharun L, Shostak K, Hermand D, Delaunay S, Klevernic I, Jiang Z, Jacques N, Jamart D, Migeot V, Florin A, Göktuna S, Malgrange B, Sansom OJ, Nguyen L, Büttner R, Close P, Chariot A.
J Exp Med. 2017 Apr 3;214(4):1199. doi: 10.1084/jem.2014228803082017c. Epub 2017 Mar 10. No abstract available.
PMID: 28283535

ALK(G1269A) mutation as a potential mechanism of acquired resistance to crizotinib in an ALK-rearranged inflammatory myofibroblastic tumor

Michels SYF, Scheel AH, Wündisch T, Heuckmann JM, Menon R, Puesken M, Kobe C, Pasternack H, Heydt C, Scheffler M, Fischer R, Schultheis AM, Merkelbach-Bruse S, Heukamp L, Büttner R, Wolf J.
NPJ Precis Oncol. 2017 Mar 20;1(1):4. doi: 10.1038/s41698-017-0004-3. eCollection 2017.
PMID: 29872693

Characterization of mast cell populations using different methods for their identification.

Atiakshin D, Samoilova V, Buchwalow I, Boecker W, Tiemann M.
Histochem Cell Biol. 2017 Jun;147(6):683-694. doi: 10.1007/s00418-017-1547-7. Epub 2017 Feb 27.
PMID: 28243739

Oxidative stress and NO generation in the rat pancreatitis induced by pancreatic duct ligation.

Buchwalow I, Schnekenburger J, Atiakshin D, Samoilova V, Wolf E, Boecker W, Tiemann K.
Acta Histochem. 2017 Apr;119(3):252-256. doi: 10.1016/j.acthis.2017.01.010. Epub 2017 Feb 3.
PMID: 28168994

Immunohistochemical and molecular profile of salivary gland cancer in children.

Locati LD, Collini P, Imbimbo M, Barisella M, Testi A, Licitra LF, Löning T, Tiemann K, Quattrone P, Bimbatti E, Chiaravalli S, Casanova M, Tamborini E, Carta R, Gasparini P, Guzzo M, Massimino M, Ferrari A.
Pediatr Blood Cancer. 2017 Jan 31. doi: 10.1002/pbc.26468. [Epub ahead of print]
PMID: 28139061

The GSK461364 PLK1 inhibitor exhibits strong antitumoral activity in preclinical neuroblastoma models

Pajtler KW, Sadowski N, Ackermann S, Althoff K, Schönbeck K, Batzke K, Schäfers S, Odersky A, Heukamp L, Astrahantseff K, Künkele A, Deubzer HE, Schramm A, Sprüssel A, Thor T, Lindner S, Eggert A, Fischer M, Schulte JH.
Oncotarget. 2017 Jan 24;8(4):6730-6741. doi: 10.18632/oncotarget.14268.
PMID: 28036269

Predictive PD-L1 immunohistochemistry for non-small cell lung cancer : Current state of the art and experiences of the first German harmonization study.

Scheel AH, Dietel M, Heukamp LC, Jöhrens K, Kirchner T, Reu S, Rüschoff J, Schildhaus HU, Schirmacher P, Tiemann M, Warth A, Weichert W, Fischer RN, Wolf J, Büttner R.
Pathologe. 2016 Nov;37(6):557-567. Review. German.
PMID: 27510417

Harmonized PD-L1 immunohistochemistry for pulmonary squamous-cell and adenocarcinomas.

Scheel AH, Dietel M, Heukamp LC, Jöhrens K, Kirchner T, Reu S, Rüschoff J, Schildhaus HU, Schirmacher P, Tiemann M, Warth A, Weichert W, Fischer RN, Wolf J, Buettner R.
Mod Pathol. 2016 Oct;29(10):1165-72. doi: 10.1038/modpathol.2016.117. Epub 2016 Jul 8.
PMID: 27389313

BCL9L expression in pancreatic neoplasia with a focus on SPN: a possible explanation for the enigma of the benign neoplasia.

Hallas C, Phillipp J, Domanowsky L, Kah B, Tiemann K.
BMC Cancer. 2016 Aug 18;16:648. doi: 10.1186/s12885-016-2707-1.
PMID: 27539223

Harmonized PD-L1 immunohistochemistry for pulmonary squamous-cell and adenocarcinomas.

Scheel AH, Dietel M, Heukamp LC, Jöhrens K, Kirchner T, Reu S, Rüschoff J, Schildhaus HU, Schirmacher P, Tiemann M, Warth A, Weichert W, Fischer RN, Wolf J, Buettner R.
Mod Pathol. 2016 Oct;29(10):1165-72. doi: 10.1038/modpathol.2016.117. Epub 2016 Jul 8.
PMID: 27389313

Integrated Analysis of PTEN and p4EBP1 Protein Expression as Predictors for pCR in HER2-Positive Breast Cancer.

Loibl S, Darb-Esfahani S, Huober J, Klimowicz A, Furlanetto J, Lederer B, Hartmann A, Eidtmann H, Pfitzner B, Fasching PA, Tiemann K, Jackisch C, Mehta K, von Minckwitz G, Untch M, Denkert C.
Clin Cancer Res. 2016 Jun 1;22(11):2675-83. doi: 10.1158/1078-0432.CCR-15-0965. Epub 2016 Jan 12.
PMID: 26758558

The contribution of Paul Ehrlich to histochemistry: a tribute on the occasion of the centenary of his death.

Buchwalow I, Boecker W, Tiemann M.
Virchows Arch. 2015 Jan;466(1):111-6. doi: 10.1007/s00428-014-1677-4. Epub 2014 Oct 31.
PMID: 25359602

The contribution of Paul Ehrlich to histochemistry: a tribute on the occasion of the centenary of his death.

Buchwalow I, Boecker W, Tiemann M.
Virchows Arch. 2015 Jan;466(1):111-6. doi: 10.1007/s00428-014-1677-4. Epub 2014 Oct 31.
PMID: 25359602

Squamous/epidermoid differentiation in normal breast and salivary gland tissues and their corresponding tumors originate from p63/K5/14-positive progenitor cells.

Boecker W, Stenman G, Loening T, Andersson MK, Berg T, Lange A, Bankfalvi A, Samoilova V, Tiemann K, Buchwalow I.
Virchows Arch. 2015 Jan;466(1):21-36. doi: 10.1007/s00428-014-1671-x. Epub 2014 Oct 26.
PMID: 25344874

Final safety and efficacy analysis of a phase I/II trial with imatinib and vinorelbine for patients with metastatic breast cancer.

Maass N, Schem C, Bauerschlag DO, Tiemann K, Schaefer FW, Hanson S, Muth M, Baier M, Weigel MT, Wenners AS, Alkatout I, Bauer M, Jonat W, Mundhenke C.
Oncology. 2014;87(5):300-10. doi: 10.1159/000365553. Epub 2014 Aug 26.
PMID: 25171229

Molekularpathologie des Lungenkarzinoms

Tiemann M, Hallas C, Falk M.
Trillium Krebsmedizin 2014, 23:6

Significance of syndecan-1 expression in ductal carcinoma in situ of the breast.

Tiemann K, Weigel MT, Alkatout I, Wenners AS, Mundhenke H, Schäfer FW, Bauer M, Schem C, Maass N, Jonat W, Mundhenke C.
Anticancer Res. 2014 Jul;34(7):3607-16.
PMID: 24982376

Differentiation and histogenesis of syringomatous tumour of the nipple and low-grade adenosquamous carcinoma: evidence for a common origin

W. Boecker, G. Stenman, T. Loening, M.K. Andersson, H.P. Sinn, P. Barth, F. Oberhellmann, I. Bos, T. Berg, Z. Marusic, V. Samoilova, I. Buchwalow
Histopathology, 65 (2014) 9-23

Signal amplification in immunohistochemistry: loose-jointed deformable heteropolymeric HRP conjugates vs. linear polymer backbone HRP conjugates.

Buchwalow I, Boecker W, Wolf E, Samoilova V, Tiemann M.
Acta Histochem. 2013 Jul;115(6):587-94. doi: 10.1016/j.acthis.2012.12.008. Epub 2013 Jan 23.
PMID: 23351717

L-arginine-NO-cGMP signalling pathway in pancreatitis

Buchwalow I, Schnekenburger J, Tiemann K, Samoilova V, Bankfalvi A, Poremba C, Schleicher C, Neumann J, Boecker W.
Sci Rep. 2013 May 28;3:1899. doi: 10.1038/srep01899.
PMID: 23712581

K5/K14-positive cells contribute to salivary gland-like breast tumors with myoepithelial differentiation

Boecker W, Stenman G, Loening T, Andersson MK, Bankfalvi A, von Holstein S, Heegaard S, Lange A, Berg T, Samoilova V, Tiemann K, Buchwalow I.
Mod Pathol. 2013 Apr 5. doi: 10.1038/modpathol.2013.45. [Epub ahead of print]
PMID: 23558567

Signal amplification in immunohistochemistry: Loose-jointed deformable heteropolymeric HRP conjugates vs. linear polymer backbone HRP conjugates

Buchwalow I, Boecker W, Wolf E, Samoilova V, Tiemann M.
Acta Histochem. 2013 Jan 23. doi:pii: S0065-1281(12)00183-3. 10.1016/j.acthis. 2012.12.008.
PMID: 23351717

Molekularpathologische Grundlagen für die Therapie des NSCLC

Die „stille Revolution“ in der Behandlung solider Tumoren. Falk M, Hallas C, Tiemann M.
best practice onkologie 2012 February 1:4
DOI: 10.1007/s11654-011-0335-4. ISSN: 0946-4565.

Origin and differentiation of breast nipple syringoma

Boecker W, Junkers T, Reusch M, Buerger H, Korsching E, Metze D, Decker T, Loening T, Lange A, Samoilova V, Buchwalow I.
Sci Rep. 2012;2:226. doi: 10.1038/srep00226. Epub 2012 Jan 17. No abstract available.
PMID: 22355740

Non-specific binding of antibodies in immunohistochemistry: fallacies and facts

Buchwalow I, Samoilova V, Boecker W, Tiemann M.
Sci Rep. 2011;1:28. doi: 10.1038/srep00028. Epub 2011 Jul 1.
PMID: 22355547

Immunohistochemistry: Basics and Methods

I.B. Buchwalow, W. Boecker
1 ed., Springer, Heidelberg, Dordrecht, London, New York, 2010

The role of arterial smooth muscle in vasorelaxation

I.B. Buchwalow, S. Cacanyiova, J. Neumann, V.E. Samoilova, W. Boecker, F. Kristek
Biochem Biophys Res Commun, 377 (2008) 504-507

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